Navigate Up

Orthopaedics Center - A-Z Index

#
I
Q
Y
Z

Print This Page

Protein electrophoresis - urine

A urine protein electrophoresis is a test that estimates how much of certain proteins you have in your urine.

See also:

Alternative Names

Urine protein electrophoresis; UPEP

How the test is performed

A clean-catch (midstream) urine sample is needed.

Men or boys should first wipe clean the head of the penis. Women or girls need to wash the area between the lips of the vagina with soapy water and rinse well.

As you start to urinate, allow a small amount to fall into the toilet bowl (this clears the urethra of contaminants). Then, in a clean container, catch about 1 to 2 ounces of urine and remove the container from the urine stream. Give the container to the health care provider or assistant.

In infants, thoroughly wash the area around the urethra. Open a urine collection bag (a plastic bag with an adhesive paper on one end), and place it on your infant. For boys, the entire penis can be placed in the bag and the adhesive attached to the skin. For girls, the bag is placed over the labia. Place a diaper over the infant (bag and all).

Check your baby frequently and remove the bag after the infant has urinated into it. For active infants, this procedure may take a couple of attempts -- lively infants can displace the bag. The urine is drained into a container for transport back to the health care provider.

The laboratory specialist will place the urine sample on special paper and apply an electric current. The various proteins move and form visible bands, which reveal the general amounts of each protein.

How to prepare for the test

Your health care provider may tell you to stop taking certain medicines that could interfere with the test. Medicines that can affect test results include:

  • Chlorpromazine
  • Corticosteroids
  • Isoniazid
  • Neomycin
  • Phenacemide
  • Salicylates
  • Sulfonamides
  • Tolbutamide

Never stop taking any medication without first talking to your health care provider.

How the test will feel

This test involves only normal urination. There is no discomfort.

Why the test is performed

Only small amounts of protein are normally found in the urine. The presence of protein in the urine can be a sign of many different disorders.

Urine protein electrophoresis may be recommended to help determine the cause of protein in the urine, or as a screening test to measure the various proteins in urine. Urine protein electrophoresis detects two types of protein: albumin and globulins.

Normal Values

No significant amount of globulins in the urine. Urine albumin is less than 5 mg/dL.

Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.

What abnormal results mean

What the risks are

There are no risks associated with this test.

References

McPherson RA, Massey HD. Laboratory evaluation of immunoglobulin function and humoral immunity. In McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia,Pa: Saunders Elsevier; 2011:chap 46.

Perry MC. Plasma cell disorders. In Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, PA: Saunders Elsevier; 2011:chap193.

Updated: 6/5/2012

David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine. Yi-Bin Chen, MD, Leukemia/Bone Marrow Transplant Program, Massachusetts General Hospital. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M. Health Solutions, Ebix, Inc.


©  UPMC | Affiliated with the University of Pittsburgh Schools of the Health Sciences
Supplemental content provided by A.D.A.M. Health Solutions. All rights reserved.

For help in finding a doctor or health service that suits your needs, call the UPMC Referral Service at 412-647-UPMC (8762) or 1-800-533-UPMC (8762). Select option 1.

UPMC is an equal opportunity employer. UPMC policy prohibits discrimination or harassment on the basis of race, color, religion, ancestry, national origin, age, sex, genetics, sexual orientation, marital status, familial status, disability, veteran status, or any other legally protected group status. Further, UPMC will continue to support and promote equal employment opportunity, human dignity, and racial, ethnic, and cultural diversity. This policy applies to admissions, employment, and access to and treatment in UPMC programs and activities. This commitment is made by UPMC in accordance with federal, state, and/or local laws and regulations.

Medical information made available on UPMC.com is not intended to be used as a substitute for professional medical advice, diagnosis, or treatment. You should not rely entirely on this information for your health care needs. Ask your own doctor or health care provider any specific medical questions that you have. Further, UPMC.com is not a tool to be used in the case of an emergency. If an emergency arises, you should seek appropriate emergency medical services.

For UPMC Mercy Patients: As a Catholic hospital, UPMC Mercy abides by the Ethical and Religious Directives for Catholic Health Care Services, as determined by the United States Conference of Catholic Bishops. As such, UPMC Mercy neither endorses nor provides medical practices and/or procedures that contradict the moral teachings of the Roman Catholic Church.

© UPMC
Pittsburgh, PA, USA UPMC.com