Navigate Up

Women's Center - A-Z Index

#
Y

Print This Page

Immunofixation - urine

Urine immunofixation is a test to look for proteins in urine.


How the test is performed

You will need to supply a clean-catch (midstream) urine sample.

  • Clean the area around where urine leaves the body. Men or boys should wipe the head of the penis. Women or girls should wash the area between the lips of the vagina with soapy water and rinse well.
  • Allow a small amount to fall into the toilet bowl as you start to urinate. This clears substances that may contaminate the sample. Catch about 1 to 2 ounces of urine in the clean container that you are given.
  • Remove the container from the urine stream.
  • Give the container to the health care provider or assistant.

For an infant:

  • Thoroughly wash the area where the urine exits the body.
  • Open a urine collection bag (a plastic bag with an adhesive paper on one end).
  • For males, place the entire penis in the bag and attach the adhesive to the skin.
  • For females, place the bag over the labia.
  • Diaper as usual over the secured bag.

I may take more than one try to get a sample from an infant. An active baby can move the bag, so that the urine goes into the diaper. Check the infant often and change the bag after the urine has been collected. Drain the urine from the bag into the container given to you by your health care provider.

Deliver the sample to the lab or your health care provider as soon as possible after it is done.

How to prepare for the test

No special steps are necessary for this test.

How the test will feel

The test involves only normal urination. There is no discomfort.

Why the test is performed

This test is most often used to check the levels of certain proteins (called monoclonal immunoglobulins). These proteins are linked to multiple myeloma and Waldenstrom's macroglobulinemia .

Normal Values

No presence of monoclonal immunoglobulins is normal.

What abnormal results mean

The presence of monoclonal proteins may indicate:

  • Immune system disorders such as multiple myeloma or Waldenstrom's macroglobulinemia
  • Cancer

Special considerations

Immunofixation is similar to urine immunoelectrophoresis , but it may give more rapid results.

References

McPherson RA, Massey HD. Laboratory Evaluation of Immunoglobulin Function and Humoral Immunity. In: Mcpherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods.22nd ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 46

McPherson RA and Pincus MR. Henry's Clinical Diagnosis and Management by Laboratory Methods.21st ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 46.

Hoffman R, Benz Jr. EJ, Shattil SJ, et al., eds. Hematology: Basic Principles and Practice. 4th ed. Philadelphia, Pa: Churchill Livingston; 2005:727-33.

Updated: 1/22/2013

Todd Gersten, MD, Hematology/Oncology, Palm Beach Cancer Institute, West Palm Beach, FL. Review provided by VeriMed Healthcare Network.Also reviewed by A.D.A.M. Health Solutions, Ebix, Inc., Editorial Team: David Zieve, MD, MHA, Bethanne Black, Stephanie Slon, and Nissi Wang.


©  UPMC | Affiliated with the University of Pittsburgh Schools of the Health Sciences
Supplemental content provided by A.D.A.M. Health Solutions. All rights reserved.

For help in finding a doctor or health service that suits your needs, call the UPMC Referral Service at 412-647-UPMC (8762) or 1-800-533-UPMC (8762). Select option 1.

UPMC is an equal opportunity employer. UPMC policy prohibits discrimination or harassment on the basis of race, color, religion, ancestry, national origin, age, sex, genetics, sexual orientation, marital status, familial status, disability, veteran status, or any other legally protected group status. Further, UPMC will continue to support and promote equal employment opportunity, human dignity, and racial, ethnic, and cultural diversity. This policy applies to admissions, employment, and access to and treatment in UPMC programs and activities. This commitment is made by UPMC in accordance with federal, state, and/or local laws and regulations.

Medical information made available on UPMC.com is not intended to be used as a substitute for professional medical advice, diagnosis, or treatment. You should not rely entirely on this information for your health care needs. Ask your own doctor or health care provider any specific medical questions that you have. Further, UPMC.com is not a tool to be used in the case of an emergency. If an emergency arises, you should seek appropriate emergency medical services.

For UPMC Mercy Patients: As a Catholic hospital, UPMC Mercy abides by the Ethical and Religious Directives for Catholic Health Care Services, as determined by the United States Conference of Catholic Bishops. As such, UPMC Mercy neither endorses nor provides medical practices and/or procedures that contradict the moral teachings of the Roman Catholic Church.

© UPMC
Pittsburgh, PA, USA UPMC.com