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Pitt Study Explains Why Adults’ Hearts Don’t Regenerate

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10/24/2022

PITTSBURGH — As heart cells mature in mice, the number of communication pathways called nuclear pores dramatically decreases, according to new research from University of Pittsburgh and UPMC scientists. While this might protect the organ from damaging signals, it could also prevent adult heart cells from regenerating, the researchers found.
 
Bernhard Kuhn releaseThe study, published today in Developmental Cell, suggests that quieting communication between heart cells and their environment protects this organ from harmful signals related to stresses such as high blood pressure, but at the cost of preventing heart cells from receiving signals that promote regeneration.
 
“This paper provides an explanation for why adult hearts do not regenerate themselves, but newborn mice and human hearts do,” said senior author Bernhard Kühn, M.D., professor of pediatrics and director of the Pediatric Institute for Heart Regeneration and Therapeutics at Pitt School of Medicine and UPMC Children’s Hospital of Pittsburgh. “These findings are an important advance in fundamental understanding of how the heart develops with age and how it has evolved to cope with stress.”
 
While skin and many other tissues of the human body retain the ability to repair themselves after injury, the same isn’t true of the heart. During human embryonic and fetal development, heart cells undergo cell division to form the heart muscle. But as heart cells mature in adulthood, they enter a terminal state in which they can no longer divide.
 
To understand more about how and why heart cells change with age, Kühn teamed up with fellow Pitt researchers and biomedical imaging experts Yang Liu, Ph.D., associate professor of medicine and bioengineering, and Donna Stolz, Ph.D., associate professor of cell biology and pathology and associate director of the Center for Biologic Imaging, to look at nuclear pores. These perforations in the lipid membrane that surround a cell’s DNA regulate the passage of molecules to and from the nucleus.
 
Yang Liu release“The nuclear envelope is an impermeable layer that protects the nucleus like asphalt on a highway,” said Kühn, who is also a member of the McGowan Institute for Regenerative Medicine. “Like manholes in this asphalt, nuclear pores are pathways that allow information to get through the barrier and into the nucleus.”
 
Using super-resolution microscopy, Liu visualized and counted the number of nuclear pores in mouse heart cells, or cardiomyocytes. The number of pores decreased by 63% across development, from an average of 1,856 in fetal cells to 1,040 in infant cells to just 678 in adult cells. These findings were validated by Stolz who used electron microscopy to show that nuclear pore density decreased across heart cell development.
 
In previous research, Kühn and his team showed that a gene called Lamin b2, which is highly expressed in newborn mice but declines with age, is important for cardiomyocyte regeneration.
 
In the new study, they show that blocking expression of Lamin b2 in mice led to a decrease in nuclear pore numbers. Mice with fewer nuclear pores had diminished transport of signaling proteins to the nucleus and decreased gene expression, suggesting that reduced communication with age may drive a decrease in cardiomyocyte regenerative capacity.
 
Donna Stolz release“These findings demonstrate that the number of nuclear pores controls information flux into the nucleus,” explained Kühn. “As heart cells mature and the nuclear pores decrease, less information is getting to the nucleus.”
 
In response to stress such as high blood pressure, a cardiomyocyte’s nucleus receives signals that modify gene pathways, leading to structural remodeling of the heart. This remodeling is a major cause of heart failure.
 
The researchers used a mouse model of high blood pressure to understand how nuclear pores contribute to this remodeling process. Mice that were engineered to express fewer nuclear pores showed less modulation of gene pathways involved in harmful cardiac remodeling. These mice also had better heart function and survival than their peers with more nuclear pores.
 
“We were surprised at the magnitude of the protective effect of having fewer nuclear pores in mice with high blood pressure,” said Kühn. “However, having fewer communication pathways also limits beneficial signals such as those that promote regeneration.”
 
Nuclear Pores releaseOther authors who contributed to this study were Lu Han, Ph.D., Jocelyn D. Mich-Basso, B.S., M.T., Yao Li, Ph.D., Niyatie Ammanamanchi, M.S., Jianquan Xu, Ph.D., Anita P. Bargaje, B.S., Honghai Liu, Ph.D., Liwen Wu, Ph.D., Jong-Hyeon Jeong, Ph.D., Jonathan Franks, M.S., Yijen L. Wu, Ph.D., and Dhivyaa Rajasundaram, Ph.D., all of Pitt or UPMC.
 
This research was supported by the Richard King Mellon Foundation Institute for Pediatric Research (UPMC Children’s Hospital of Pittsburgh), HeartFest, the National Institutes of Health (R01HL151415, R01 HL151386, R01HL155597, T32HL129949, EB023507 and NS121706-01), the American Heart Association (18CDA34140024), and the U.S. Department of Defense (W81XWH1810070 and W81XWH-22-1-0221), the Clinical and Translational Science Institute at Pitt, and the Aging Institute at Pitt and UPMC.

Top photo:

PHOTO DETAILS: (click images for high-res versions)

CREDIT: UPMC

CAPTION: Bernhard Kühn, M.D., professor of pediatrics and director of the Pediatric Institute for Heart Regeneration and Therapeutics at Pitt School of Medicine and UPMC Children’s Hospital of Pittsburgh

 

Second photo:

PHOTO DETAILS: (click images for high-res versions)

CREDIT: UPMC

CAPTION: Yang Liu, Ph.D., associate professor of medicine and bioengineering at Pitt

 

Third photo:

PHOTO DETAILS: (click images for high-res versions)

CREDIT: Donna Stolz

CAPTION: Donna Stolz, Ph.D., associate professor of cell biology and pathology at Pitt and associate director of the Center for Biologic Imaging

 

Bottom image:

PHOTO DETAILS: (click images for high-res versions)

CREDIT: Han et al., 2022, Developmental Cell, 10.1016/j.devcel.2022.09.017

CAPTION: Electron microscopy images of fetal (left) and infant (right) rodent heart cell nuclei. As heart cells develop, the number of nuclear pores decreases.